Fluorescent reporter libraries as useful tools for optimizing microbial cell factories: a review of the current methods and applications

摘要

The use of genetically encoded fluorescent reporters allows speeding up the initial optimizationsteps of microbial bioprocesses. These reporters can be used for determiningthe expression level of a particular promoter, not only the synthesis of a specific proteinbut also the content of intracellular metabolites. The level of protein/metabolite is thusproportional to a fluorescence signal. By this way, mean expression profiles of protein/metabolites can be determined non-invasively at a high-throughput rate, allowing therapid identification of the best producers. Actually, different kinds of reporter systemsare available, as well as specific cultivation devices allowing the on-line recording of thefluorescent signal. Cell-to-cell variability is another important phenomenon that can beintegrated into the screening procedures for the selection of more efficient microbial cellfactories.